The study on the formation mechanisms of nanocomposite functional films and new deployment of metal works by an application thereof.

为了更好地理解血红素加氧酶（HO）-1作为细胞对非热低强度脉冲超声（LIPUS，0.3 W/cm 2，1 min）和轻度热疗（MH，41 ℃，30 min）反应的一个组成部分的分子机制，我们使用高密度寡核苷酸微阵列和计算基因表达分析来检测人类淋巴瘤U937细胞的基因表达模式和遗传网络。LIPUS处理后6小时，观察到细胞凋亡（14±3.8%），无细胞溶解。在分析的22，283个探针组中，LIPUS下调了193个基因，上调了201个基因，上调幅度>1.5倍。另一方面，423个基因表达上调，515个基因表达下调的细胞与MH处理后3小时，不诱导凋亡。在LIPUS和MH处理的细胞中，HO-1与NRG 1或NF 2以及BCL 2或CREB 1之间存在相互作用，并确定了重要的遗传网络。当细胞受到X射线照射时，HO-1在17937细胞中表达上调，而在HeLa细胞中没有表达上调，本研究结果表明，LIPUS和MH影响许多基因的表达，并为HO-1的生物分子机制提供了新的见解。与HO-1相互作用的辐射响应基因的鉴定仍然是未来研究的主题。

For better understanding of the molecular mechanism of hemeoxygenase(HO)-1 as a component of cellular response to non-thermal low intensity pulsed ultrasound(LIPUS, 0.3 W/cm2 for 1 min), and mild hyperthermia (MH, 41 0C, 30 min), we examined gene expression patterns and genetic networks in human lymphoma U937 cells using high-density oligonucleotide microarrays and computational gene expression analyses. Six hours after LIPUS treatment, apoptosis(14±3.8%) with no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. On the other hand, 423 genes were up-regulated and 515 genes were down-regulated in the cells 3 h post-treatment with MH which did not induce apoptosis. The significant genetic networks were identified and the interaction between HO-1 and NRG1 or NF2, and BCL2 or CREB1 was observed in the cells treated with LIPUS and MH, respectively. When the cells were exposed to X-rays, up-regulation of HO-1 was observed in 17937 cells but not in HeLa cells.The present results indicate that LIPUS and MH affect the expression of many genes and provide novel insight into the biomolecular mechanisms of HO-1. The identification of radiation-responsive genes interacting with HO-1 remains a subject for future studies.