Genital tract malformation and angiogenesis in the female mice exposed to diethylstilbestrol neonatally

1. 基质金属蛋白酶（Matrix metalloproteinase， MMP）的表达通过RT-PCR检测发现，10日龄新生小鼠（des小鼠）子宫中MMP-2和MMP-9 mRNA的表达较对照小鼠明显降低。免疫组化研究显示，des小鼠子宫内MMP-2表达明显降低。使用荧光标记明胶的原位酶谱法，在两个实验组的子宫中均未检测到MMP-2的酶活性，尽管des小鼠子宫中的粒细胞显示MMP-9酶活性增加。组织金属蛋白酶抑制剂（Tissue inhibitor of metalloproteinase， TIMP）表达rt - pcr结果显示，对照组与des小鼠的TIMP-1、-2、-3、-4 mRNA表达水平无显著差异。胶原表达通过rt - pcr发现，对照组和des小鼠子宫内i型、-III型和-IV型胶原mRNA表达几乎相同。血管内皮生长因子（VEGF）的表达免疫组化研究显示，血管内皮生长因子在子宫间质细胞中的表达明显低于对照组。

1. Matrix metalloproteinase (MMP) expressionThe mRNA expression of MMP-2 and MMP-9 was shown to decrease by using RT-PCR in the uteri of 10-day-old mice exposed to diethylstilbestrol neonatally (DES-mice)than those in vehicle-treated control mice. Immunohistochemical study demonstrated that MMP-2 expression significantly decreased in the uteri of DES-mice. In situ zymography using fluorescence-labeled gelatin, enzyme activity of MMP-2 was not detected in the uteri of both experimental groups, although granulocytes showing MMP-9 enzyme activity increased in the DES-mice uteri.2. Tissue inhibitor of metalloproteinase (TIMP)expressionRT-PCR showed that no significant differences in the mRNA levels of TIMP-1, -2, -3 and -4 between the control and DES-mice.3. Collagen expressionThe mRNA expression of collagen type-I, -III and -IV was shown to be almost the same in uteri of the control and DES-mice by using RT-PCR.4. Vascular endothelial growth factor (VEGF) expressionImmunohistoichemical study showed that VEGF expression in the uterine stromal cells was much lower level than in those of controls.