Role of plasma S19 ribosomal protein in thrombus resorption

为了阐明血浆和单核细胞中存在的S19核糖体蛋白在血栓吸收中的作用，我们建立了一个实验模型，将预先形成的凝血移植到豚鼠腹腔。移植后1天内，凝块表面被单核/巨噬细胞完全覆盖。凝块上的单核/巨噬细胞相互连接形成上皮样结构。第3天凝块大小迅速减小，第7天消失。我们在家兔中培养了抗s19核糖体蛋白抗体。将预形成的凝血液离心提取血清，血清中恢复了一种单核细胞定向趋化因子。单核细胞趋化因子经免疫学鉴定为S19核糖体蛋白二聚体。将豚鼠血液在抗s19核糖体蛋白抗体存在下凝固后移植入腹腔，吸收过程中上述事件均明显减少。特别是，凝固表面仅部分被单核/巨噬细胞覆盖，这些细胞未形成上皮样细胞。这些结果强烈提示血浆中S19核糖体蛋白被活化的凝血因子XIII交联，导致凝血过程中二聚体的形成，该二聚体募集单核细胞参与凝血吸收。

To elucidate roles of S19 ribosomal protein present in plasma and monocytes in the thrombus resorption, we developed an experimental model in which pre-formed blood coagula was transplanted into the guinea pig peritoneal cavity. The surface of the coagula was fully covered by monocytes/macrophages within a day after the transplantation. The monocytes/macrophages on the coagula inter-connected each other forming an epithelia-like pattern. The size of coagula rapidly decreased by day 3 and disappeared by day 7.We raised anti-S19 ribosomal protein antibodies in rabbits. When the pre-formed blood coagula was centrifuged and serum was obtained, a monocyte-directed chemotactic factor was recovered in the serum. The monocyte chemotactic factor was immunologically identified to be the S19 ribosomal protein dimer. When guinea pig blood was coagulated under the presence of the anti-S19 ribosomal protein antibodies and then transplanted into the peritoneal cavity, all of the above events in the resorption process were significantly reduced. Especially, the coagula surface was covered only partly by monocytes/macrophages and the epithelia-like formation of these cells was not seen.These results strongly suggested that the S19 ribosomal protein in plasma was cross-linked by activated coagulation factor XIII resulting in the dimer formation during blood coagulation and that this dimer recruited monocytes which would involve in the coagula resorption.