Molecular mechanisms of stem cell self-renewal.

肌肉的生长和再生严重依赖于其干细胞区室。我们已经发现，p38 MAPK途径是必不可少的干细胞自我更新的C2C12肌细胞系。该提案旨在了解骨骼肌中干细胞自我更新的分子基础，可能适用于许多干细胞系统的数据，以及干细胞治疗老年人损伤和疾病的巨大前景。我们将尝试通过强制p38表达来改变干细胞生产的平衡，并采用微阵列和蛋白质组学方法来定义干细胞通路。

Muscle growth and regeneration is critically dependent on its stem cell compartment. We have discovered that the p38 MAPK pathway is essential for stem cell self-renewal in the C2C12 myogenic cell line. This proposal seeks to understand the molecular basis of stem cell self-renewal in skeletal muscles, data that may be applicable to many stem cell systems, and to the enormous promise of stem cell therapies for injury and diseases of the aged. We will attempt to alter the balance of stem cell production by enforced p38 expression, and take microarray and proteomics approaches to define stem cell pathways.