The in vitro and in vivo study on effects of growth factor on tooth germs

本研究在A组(对照组)、B组(二甲基亚砜)、C组(转化生长因子-P-2)、D组(转化生长因子-P-2)和E组(转化生长因子-α+转化生长因子-P-2)共5组小鼠，观察了生长因子转化生长因子-α和致癌物Trp-P-2对移植到裸鼠皮下的牙胚发育的影响。组织学观察，C、D、E组牙源性上皮细胞明显增殖。D组和E组牙源性上皮细胞角质化，C组未见角质化；E组牙源性上皮细胞区可见囊样间隙。免疫组织化学显示，C、D、E组牙源性上皮细胞的增殖细胞核抗原阳性率明显高于A组。组织计量学显示，C、E组牙胚的大小明显大于Gro…本研究结果表明，生长因子转化生长因子α-α和致癌物Trp-P-2可促进小鼠器官培养牙胚上皮细胞的增殖、角质化和囊样间隙的形成。本研究观察了致癌物MeIQx和生长因子转化生长因子转化生长因子-α对小鼠牙胚发育的影响。D组(MeIQx)和E组(MeIQx+转化生长因子-α)。组织学上，A组和B组的牙胚大小相似。C组牙胚体积大于A、B组，D、E组牙胚小于C组。C、D、E组牙源性上皮细胞明显增殖。免疫组织化学显示，B、C、F、G组牙源性上皮细胞增殖细胞核抗原阳性率明显高于A组。本研究结果提示，致癌物MeIQx和生长因子转化生长因子α对小鼠器官培养牙胚中牙源性上皮细胞的增殖有促进作用。取17日龄ddy小鼠胚胎的牙胚，按其培养条件分为5组：a组(对照组)、b组(二甲基亚砜)、c组(转化生长因子-α)、d组(转化生长因子-α)、e组(转化生长因子-DNA+转化生长因子)。将这些牙胚移植到裸鼠皮下组织中，移植2周后，对移植的器官培养的牙胚进行组织学和免疫组织化学(增殖细胞核抗原染色)检测。C、d、e组移植的器官培养牙胚中可见牙源性上皮细胞增殖。C组和e组牙源性上皮细胞区可见囊样间隙。S组和e组牙源性上皮细胞增殖细胞核抗原阳性率明显高于A组。本研究结果表明，转化生长因子-α和ENU对牙胚移植后牙源性上皮细胞的增殖有明显的促进作用

I. In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ which was transplanted into the subcutaneous tissue of nude mice were investigated histologically, immnohistochemically (PCNA) and histometrically in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α + Trp-P-2). Histologically, in Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C.Moreover, in Group E, a cyst-like space was observed in the area of odontogenic epithelial cells. Immunohistochemically, in Groups C, D and E, PCNA-positivities of the odontogenic epithelial cells were significantly higher than those of Group A.Histometrically, in Groups C and E, the size of the tooth germ was significantly larger in size than that of Gro … More up A. The results of the present study suggest that proliferation, keratinization, cyst-like space formation of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice were promoted by growth factor TGF-α and carcinogen Trp-P-2.II. In the present study, the effects of both carcinogen MeIQx and growth factor TGF-α on the development of organ-cultured ddY mouse tooth-germs were investigated histologically and immnohistochemically (PCNA) in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (MeIQx) and Group E (MeIQx + TGF-α). Histologically, in Groups A and B, both tooth-germs appeared in similar size. In Group C, the tooth-germ was bigger in size than those of Groups A and B. In Groups D and E, the tooth-germs were smaller than in Group C. In Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. Immunohistochemically, in Groups B, C, F and G, PCNA-positivities of odontogenic epithelial cells were significantly higher than those in Group A. The results of the present study suggested that the proliferation of odontogenic epitherial cells in organ-cultured tooth-germs in mice was promoted by carcinogen MeIQx and growth factor TGF-α.III. The purpose of the present study was to investigate the effects of growth factor TGF-α and carcinogenen ENU on the development of the transplanted organ-cultured tooth germs in ddY mice. The organ-cultured tooth-germs removed from the 17th day embryo of the ddY mouse were divided into the following 5 groups according their cultured conditions, Group a (Control), Group b (DMSO), Group c (TGF-α), Group d (ENU), and Group e (TGF-α + ENU). These tooth-germs were transplanted into the subcutaneous tissue of the nude mice, and the transplanted organ-cultured tooth-germs were examined histologically and immunohistochemically (PCNA stainings) 2 weeks after transplantation. In Groups c, d and e, proliferation of odontogenic epithelial cells was found in the transplanted organ-cultured tooth-germ. Moreover, in Groups c and e, a cyst-like space was observed in the area of odontogenic epithelial cells. In groups s and e, PCNA-positivities of odontogenic epithelial cells were significantly higherthan that of Group a. The results of the present study indicated that TGF-α and ENU promoted markedly proliferation of odontogenic epithelial cells in the transplanted organ-cultured tooth-germ Less