High functional nanobio surface modification of titanium implant with bone morphogenetic protein

本研究的目的是探讨磷酸化氨基酸和磷酸化肽在钛表面的结合和解离机理，并评价利用重组人骨形态发生蛋白（BMP）-2.1对钛表面进行表面修饰的技术。表面等离子体共振（SPR）分析表明，磷酸根官能团吸附在钛-SPR传感器表面，涉及溶解表面纳米薄层的现象.采用水溶性碳化二亚胺（WSC）对钛表面进行了o-磷酸-L-丝氨酸修饰，使牛血清白蛋白组分V和重组人BMP-2在钛表面接枝聚合，从而证明了这种含磷酸基的分子和WSC对钛表面的生物化学修饰具有很好的应用前景。

The purpose of this study was to investigate a mechanism on binding and dissociation of phospho-amino acids and phospho-peptide to titanium surface, and to evaluate a surface modification technique for titanium surface using recombinant human bone morphogenetic protein (BMP) -2.1. Surface plasmon resonance (SPR) analyses clarified that phosphate functional group adsorbed to the surface of the titanium-SPR sensor involving the phenomenon to dissolve the nano-thin layer of the surface.2. An application of a water solube carbodiimide (WSC) for the surface modified with o-phospho-L-serine enabled these graft polymerization of bovine serum albumin fraction V and recombinant human BMP-2 to the surface.Consequently, it was concluded that the molecule with phosphate functional group and WSC were greatly available for biochemical surface modification process of titanium implant with recombinant human BMP-2.