A study of the clinical bacteriology of the infective endocarditis in the pedodontics by using molecular technique

1.牙科治疗和关于感染性心内膜炎（IE）相关性的问卷调查：我们对800名日本儿科学会授权的专家进行了问卷调查，以阐明牙科治疗和IE的相关性。结果，儿科医生继续对IE高度感兴趣，并且建议进行盲性抗菌剂预防的趋势与先天性心脏病儿童的出血牙科治疗的风险数量无关。IE的发病率为2.7%，不认为是由牙科治疗引起的。但在发病病例中，抗菌药物预防用药量有少量减少的趋势.口腔治疗与IE病因微生物菌群分析方法的建立：作为口腔治疗与IE病因的检测手段，我们研制了口腔治疗与IE病因微生物菌群分析方法， ...更多信息 通过DGGE方法使用杆状决定蛋白基因（rodA）基因的分析方法。本研究首先选择了人口腔中所有可能检出的高口腔链球菌型菌株27株，采用PCR方法扩增出rodA基因。直接测序法使用与我们在PCR中使用的引物相同的引物对扩增产物进行测序，并确定序列。接下来，我们分析了关于所获得的链球菌型菌株的rodA基因的遗传系统，并从GC含量计算Tm值，并确定了DNA的变性条件（尿素浓度、迁移温度），该变性条件可以在DGGE方法中检测到每个菌株的迁移模式中的差异。在此条件下，我们对所有链球菌型菌株27个原种的rodA基因扩增片段进行了测序，并对每个菌株的迁移模式进行了分析。因此，通过将扩增直接从唾液中提取的细菌DNA的基因片段的迁移模式与模板和上述模式菌株的迁移模式进行比较，能够分析唾液的微生物群。少

1.Dental treatment and questionary survey about the association of the infective endocarditis(IE) :We performed questionary survey for a specialist in 800 Japanese Society of Pedodontics authorizations to elucidate dental treatment and association of IE. As a result the pedodontist continued having interest for IE highly, and a tendency to perform the prophylaxis of the blind antimicrobial agent was suggested without being associated with the number of the risk for the bloody dental treatment of the child of the congenital heart disease. The onset of IE was 2.7% in this investigation, and it was not thought that it was caused by dentistry treatment. However; the tendency that the prophylactic dosage of the antimicrobial agent was low quantity slightly was suggested in the onset example.2. The establishment of the microbe crowd analytical method to determine dental treatment and causation of IE :As means to determine dental treatment and causation of IE, we developed the microbe crowd a … More nalytical method by DGGE method using a rod shape determining protein gene (rodA)gene. At first we chose all high oral cavity streptococcal type strain 27 stocks of detected possibility among human oral cavity and amplified a rodA gene by PCR method. A direct sequence did an amplified product using a thing same as the primer which we used in PCR, and we determined the sequence. Next time, we analyzed a heredity system about the rodA gene of obtained streptococus type strain and calculated Tm value from the GC content and determined a denaturation condition (the density of urea, migration temperature)of the DNA which a difference could detect in a migration pattern every each strain of can put it in DGGE method. We electrophoresed a rodA gene amplification section of all streptococus type strain 27 stocks under this condition and did profiling of a migration pattern every strain. Therefore, the analysis of the microbial crowd of saliva was enabled by comparing the migration pattern of the gene section that amplified the bacteria DNA which it extracted from saliva directly to a template and a migration pattern of the type strain mentioned above. Less