Analysis of proliferation and differentiation of human embryonic stem cells and research for clinical application

我们试图探索从人ES细胞中产生所需谱系特异性干细胞的培养系统，并成功地产生了造血细胞、内皮细胞、骨骼肌细胞、心肌细胞和神经细胞的祖细胞。我们建立了一种新的协议，从小鼠ES/iPS细胞与单克隆抗体SM/C-2.6，识别静止卫星细胞的肌源性前体。SM/C-2.6阳性细胞是高度肌原性的，并且在体外和体内都有效地分化成肌纤维。最近，我们开发了从人ES细胞培养骨骼肌细胞的系统，最近，我们开发了一种优秀的人源化模型小鼠NOG小鼠，在该小鼠中，即使将少量的CD 34 ^+细胞移植到小鼠中，也能成功地植入。在这项研究中，我们证实了NOG小鼠可用于评价ES/iPS细胞来源的组织特异性干细胞。

We attempt to discover the culture system for generation of desired lineage-specific stem cells from human ES cells and succeeded in generating progenitor cells for hematopoietic cells, endothelial cells, skeletal muscle, cardiomyocytes and neural cells.VEGFR-2(+)CD34(+) cells are identified as a candidate of primate hemangioblasts. We established a novel protocol to derive myogenic precursors from murine ES/iPS cells with a monoclonal antibody SM/C-2.6 that recognizes quiescent satellite cells. SM/C-2.6-positive cells are highly myogenic and efficiently differentiate into myofibers both in vitro and in vivo. We recently developed culture system for skeletal muscle cells from human ES cells.Recently, we developed an excellent humanized model mouse, NOG mouse, in which successful engraftment was achieved even if small number of CD34^+ cells were transplanted into the mice. In this study we confirmed that NOG mouse was useful for evaluation of ES/iPS cell-derived tissue specific stem cells.