Die Rolle transkriptioneller Regulation beim Start des Zellzyklus der Hefe

G1晚期进入细胞周期是真核细胞周期的一个重要调控步骤。在酵母中，进入S期的时间在很大程度上取决于细胞达到临界大小时G1期细胞周期蛋白和DNA合成基因的转录激活。它们的表达受细胞周期蛋白依赖性激酶Cdc 28 p调节，并依赖于称为SBF（Swi 4/Swi 6）和MBF（Mbp 1/Swi 6）的两种转录因子复合物。在这个项目中，我们研究了这些转录因子的功能和调控。我们已经从遗传学和生物化学上鉴定了G1晚期基因表达所需的新蛋白质（Ctr 9 p和Paf 1 p）。他们的生理作用正在分析中。该项目的目的是阐明G1晚期的转录激活如何与细胞大小和细胞生长协调。

Entry into cell cycle in late G1 is a central regulatory step of the eukaryotic cell cycle. In yeast, the timing of S-phase entry is largely determined by transcriptional activation of G1 cyclins und DNA synthesis genes as cells reach a critical size. Their expression is regulated by the cyclin dependent kinase Cdc28p and depends on two transcription factor complexes called SBF (Swi4/Swi6) and MBF (Mbp1/Swi6). In the proposed project we study the function and regulation of these transcription factors. We have genetically and biochemically identified novel proteins (Ctr9p and Paf1p) required for gene expression in late G1. Their phyiological role is being analysed. The aims of the project are to elucidate how transcriptional activation in late G1 is coordinated with cell size and cellular growth.