Development of a new mass fragmentation analysis for understanding the diversity of metabolite
Development of a new mass fragmentation analysis for understanding the diversity of metabolite
批准号:
20200062
负责人:
SUZUKI Hideyuki
金额:
$20.38万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
为了了解生物产生的代谢物的多样性,我们对代谢物的氧化反应进行了三项专题研究。1)从荷花中分离黄酮类化合物8-羟化酶cDNA。这是首次从植物中鉴定到类黄酮8-羟化酶cDNA,该酶是一种新型的类黄酮羟化酶。2)建立了在氧-18同位素和光照条件下(光照周期16 h /暗周期8 h)生长4周的日本莲花代谢产物分析体系。3)建立了基于MS/MS碎片化分析的综合代谢物网络系统,对代谢物的结构类群进行分类。
英文摘要
In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.
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DOI:
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发表时间:
2010
期刊:
影响因子:
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作者:
[]
通讯作者:
Studies on catabolic pathway of spermidine in E. coli
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Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis
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Development of a method and an educational system that support skill of"to be persuaded" based on interactive view of persuasion
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Production, Transportation and Storage of Natural Methane Hydrate
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Development of Presentation education method based on the theory of Multvoicedness
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Molecular mechanism of autocatalytic processing of γ-glutamyltranspeptidase
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Establishment of Design Procedure of Semi-submersible Type Very Large Floating Structure
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Studies on molecular mechanism of glutathione one salvage pathway of Escherichia coli
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财政年份:1998
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依托单位:
Study on protease which processes gamma-glutamyltranspeptidase
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依托单位:
Optimal Elastic Response and Design of Very Large Floating Structure
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依托单位:
Development of Automatic Installation Technology for Underwater Flexible Structures
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依托单位:
Development of Active Heave Compensator
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依托单位:
Development of Installation Technique of Flexible Ocean Structure into Deepsea
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