Analys is of exocytotic mechanism of BDNF from astrocytes.

大脑由许多细胞组成，包括神经元和神经胶质细胞。星形胶质细胞是一类神经胶质细胞，通过Ca（2+）和SNARE依赖的胞吐作用释放多种生物活性物质。在本研究中，我们发现，有效的蛋白激酶C激活剂，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）治疗抑制外源性表达的人生长激素从培养的星形胶质细胞的释放。此外，PMA处理诱导突触体相关蛋白23 kDa的Ser（95），Ser（120）和Ser（160）的磷酸化。

The brain are made up of many cells, including neurons and glial cells. Astrocytes, a type of glial cells release various bioactive substances via Ca(2+)-and SNARE-dependent exocytosis. In the present study, we revealed that potent protein kinase C activator, phorbol 12-myristate 13-acetate (PMA) treatment suppressed exogenously expressed human growth hormone release from cultured astrocytes. Moreover, PMA treatment induced the phosphorylation of synaptosomal-associated protein of 23 kDa on Ser(95), Ser(120), and Ser(160).