Analysis of molecular mechanisms of endocytosis in C.elegans development

我们分析了线虫卵母细胞卵黄摄取缺陷的rme突变体，并鉴定了从线虫到人类保守的新的rme基因。我们发现rme-3基因编码clathrin。我们还发现，RME-4在包被的小凹和/或小泡上发挥作用，招募小的GTP酶RAB-35，而RAB-35又在内体中发挥作用，促进受体的循环。我们进一步确定了Rab35效应器分子的候选分子，并检测了其在内吞作用中的功能。

We analyzed the rme mutants which show defects in yolk uptake by oocytes in C.elegans and identified novel rme genes conserved from the worm to human. We found that the rme-3 gene encodes clathrin. We also revealed that RME-4 functions on coated pits and/or vesicles to recruit small GTPase RAB-35, which in turn functions in the endosomes to promote receptor recycling. We further identified a candidate for the Rab35 effector molecule and examined its functions in endocytosis.