Dynamic analysis of transport vesicle formation using artificial planar lipid bilayer

使用人工平面脂质双层对运输囊泡形成的动态分析

• 批准号: 19687008
• 负责人: SATO Ken
• 金额: $ 16.31万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19687008/
• 关键词: 小胞輸送; 輸送小胞; 小胞体; 低分子量GTPase; 出芽酵母; 1分子計測; プロテオリポソーム; GTPase

We investigated the dynamics of fluorescently labeled cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. We show that continuous GTPase cycles of Sar1 facilitate cargo concentration into COPII vesicle buds. We propose that the minimal set of COPII components is required to concentrate cargo molecules.

我们用单分子显微镜结合人工平面脂质双层研究了荧光标记的Cargo蛋白在COPII囊泡形成过程中的动力学。我们发现，Sar1的连续GTPase循环促进了货物向COPII囊泡芽的聚集。我们提出，浓缩货物分子需要COPII组分的最小集合。

项目成果

Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3

• DOI: 10.1038/nsmb.1722
• 发表时间: 2010-02-01
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Yamashita, Masami;Kurokawa, Kazuo;Fukai, Shuya
• 通讯作者: Fukai, Shuya

Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane

• DOI: 10.1038/emboj.2009.269
• 发表时间: 2009-11-04
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Tabata, Kazuhito V.;Sato, Ken;Noji, Hiroyuki
• 通讯作者: Noji, Hiroyuki

VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana

• DOI: 10.1105/tpc.107.053876
• 发表时间: 2007-11-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Goh, Tatsuaki;Uchida, Wakana;Nakano, Akihiko
• 通讯作者: Nakano, Akihiko

Dynamics of COPII-mediated cargo concentration observed in a planar lipid membrane

在平面脂质膜中观察到的 COPII 介导的货物浓度动态

• 发表时间: 2008
• 作者: Makoto Kobayashi;et al. (9番目);Ken Sato
• 通讯作者: Ken Sato