Trial to identify membranous protein(s) regulating the planar cell polarity

已知的平面细胞极性主要是由定位于质膜上的Frizzled1受体产生的信号决定的，尽管关于Frizzled1的配基分子的现有信息仍然有限。本研究旨在利用果蝇细胞培养系统结合嵌合分子(Frizzled1-Frizzled2嵌合体)技术揭示未知的配体分子(S)。我们已经建立了基于培养细胞的靶分子检测系统，并构建了能够在果蝇中表达各种膜受体分子的结构。我们正试图调查受体分子，以揭示有问题的Frizzled1-配体。

Planar cell polarity is known to be established mainly by the signals originated from the Frizzled-1 receptor localized on the plasma membrane, although available information on the ligand molecule for Frizzled-1 is still limited. The present study has aimed to reveal the unknown ligand molecule(s) using Drosophila cell culture system coupled with chimeric molecule (Frizzled-1-Frizzled-2 chimeras) technique. We have established culture cell-based assay system of the target molecules, together with the constructs enabling to express various membranous receptor molecules reported in Drosophila. We are trying to survey receptor molecules to reveal the Frizzled-1-ligand in question.