畜産食品タンパク質をモデルとした合成ペプチドによる生体調節機能の解明

氧化应激与胃肠道的粘膜炎症有关。一些研究已经描述了来自几种动植物来源的蛋白质的抗氧化活性。蛋清蛋白、蛋黄蛋白、大豆蛋白和牛奶酪蛋白等蛋白的水解肽的抗氧化活性已有报道。最近的研究描述了蛋黄蛋白、磷高磷蛋白和磷高磷蛋白磷酸肽(PPP)的抗氧化活性。一种炎性趋化因子，白介素8(IL-8)，是由氧化应激诱导的，抗氧化剂已被证明抑制IL-8的表达。已知过氧化氢可增加人上皮细胞系中IL-8的产生。我们研究了化学合成的聚磷酸丝氨酸作为PPP模型对过氧化氢诱导的肠上皮Caco-2细胞产生IL-8的影响。采用酶联免疫吸附试验(EL ISA)检测趋化因子的产生。我们发现，合成的聚磷丝氨酸显著抑制过氧化氢诱导的Caco-2细胞中IL-8的产生。此外，这种抑制作用具有剂量依赖性。结果表明，这些磷酸肽具有减轻氧化应激所致的肠道炎症的作用。

Oxidative stress has been implicated in the mucosal inflammation of the gastrointestinal tract. Several studies have described the antioxidant activity of proteins from several animal and plant sources. The antioxidant activity of peptides generated from the hydrolysis of various proteins, such as egg white protein, egg yolk protein, soy protein, and milk casein has been reported. Recent studies have described the antioxidant activity of egg yolk protein phosvitin and phosvitin phosphopeptides (PPP). An inflammatory chemokine, interleukin-8 (IL-8), is induced by oxidative stress, and the antioxidants have been shown to inhibit IL-8 expression. Hydrogen peroxide is known to increase IL-8 production in human epithelial cell lines. We investigated the effect a chemically synthesized poly-phosphoserine as a moldel of PPP on the production of IL-8 induced by hydrogen peroxide in intestinal epithelial Caco-2 cells. The chemokine production was measured by enzyme-linked immunosorbent assay (ELISA). We found that the synthesized poly-phosphoserine significantly inhibited the hydrogen peroxide-induced IL-8 production in Caco-2 cells. Moreover, the inhibition was dose-dependent. The results suggested that the phosphopeptides have the potential to attenuate intestinal inflammation by oxidative stress.