Single molecular analysis of the nuclear membrane formation

染色体末期在染色体的中心形成称为“核区”的独特结构。在本研究中，我旨在从单分子水平上分析核的形成机制。为了解决这个问题，我研究了1)emerin的3d结构，2)染色体上的BAF结合位点，这些都是形成核心区域的关键因素。核磁共振分析表明，emerin在溶液中除了直接与BAF结合的LEM结构域外，没有形成固体结构。另一方面，尽管BAF在间期与转录阴性区结合，但它在末期与转录活性区相对结合。从这些证据中，形成核心区所需的蛋白质的分子动力学逐渐在单分子水平上被揭示。

The unique structure termed 'core region' is formed on the center of chromosome at telophase. In this study, I aimed to analyze the core formation mechanism at a single molecular level. To approach this issue, I investigated 1) the 3D-structure of emerin, and 2) a BAF binding site on the chromosomes, those of which were crucial factors to form the core region. By a nuclear magnetic resonance analysis, it was revealed that emerin did not take a solid structure in a solution except the LEM domain, which directly binds to BAF. On the other hand, BAF relatively binds to a transcription-active region at telophase although it binds to a transcription-negative region at interphase. From these evidences, the molecular dynamics of the proteins needed to form the core region are gradually uncovered at a single molecular level.