Can we explain the varying pathologic processes of rheumatoid arthritis by CaMKII delta abnormality?

我们研究了类风湿性关节炎（RA）的发病机制与CaMKII/PADIs/肽瓜氨酸化/滑膜细胞和免疫活性细胞的细胞异常之间的关系。PADIs在类风湿滑膜组织中的表达目前尚不清楚。根据我们的数据，PADIs的表达较弱，而PADIs底物存在于类风湿滑膜细胞中。类风湿性滑膜细胞中的PADIs底物可通过与邻近的免疫活性细胞相互作用而被瓜氨酸化，从而刺激RA患者的抗环瓜氨酸肽抗体（抗ccp抗体）。我们目前的数据还表明，调节性T细胞（Treg细胞），一种主要的免疫能力细胞群，在RA患者中功能下调，这可能与疾病过程的延续有关。然而，需要进一步的调查来确定这些现象是主要事件还是次要结果。此外，对Treg细胞与CaMKII/ padi的关系的研究也是必要的。在临床研究中，我们发现mri证实的骨水肿与抗ccp抗体密切相关。目前，检测肽瓜氨酸化是非常困难的。因此，我们也将通过免疫复合物分析来解决这个问题。

We have examined the relationship between the pathogenesis of rheumatoid arthritis (RA) and CaMKII/PADIs/peptide citrullination/cellular abnormality of synovial cells and immune competent cells. Expression of PADIs in the rheumatoid synovial tissues remains to be clarified at present. According to our data, the expression of PADIs was weak whereas that of PADIs substrates were present in rheumatoid synovial cells. PADIs substrates in rheumatoid synovial cells could be citrullinated by interaction with neighboring immune competent cells, resulting in stimulation of anti-cyclic citrullinated peptide antibodies (anti-CCP antibodies) in patients with RA. Our present data also suggested that the function of regulatory T cells (Treg cells), a major immune competent cell population, is down-regulated in patients with RA that may associate with perpetuation of the disease's process. However, further investigation is necessary to identify whether these phenomena are primary events or secondary results. In addition, the investigations regarding to the association of Treg cells with CaMKII/PADIs are also be necessary. In clinical study, we have identified a close association of MRI-proven bone edema with anti-CCP antibodies. At present, a detection of peptide citrullination is very difficult. Therefore, we are also going to solve this problem by immune complexsome analysis.