Clarification of mechanism of division, proliferation, differentiation, and apoptosis of supporting cells in regeneration of cochlear hair cells

在发育中的大鼠中，Caspase 3和p27抗体双染色显示，在出生后7天，Corti器官中存在大量阳性染色细胞。这些结果表明，在出生后7天，细胞凋亡被促进，增殖被停止。取出生后第7天切除的Corti器官，用1mM新霉素溶液或酸性溶液培养5 d。然后，从培养开始第3天开始给药溴脱氧尿嘧啶（BrdU）作为增殖标志物。支持细胞中未见BrdU阳性染色细胞，提示支持细胞在毛细胞缺失后3^<rd>天内未增殖。

In developing rats, double-staining with antibodies to Caspase 3 and p27 have shown that large numbers of positive staining cells exist in the organ of Corti on 7 days after birth. These findings suggest that apoptosis is promoted and that proliferation was stopped 7 days after birth. The organ of Corti extirpated on 7th day after birth was cultured with 1mM neomycin solution or acidic acid for 5 days. Then, bromodeoxyuridine (BrdU) as a proliferation marker was administered from 3rd day after starting the culture. No BrdU positive staining cell was observed in the supporting cell, suggesting that supporting cells did not proliferate up to 3^<rd> day after missing hair cell.