Development of novel method of therapy for Vibrio vulnificus infeotion unlque to surrounding area of Ariak Sea

对一株感染创伤弧菌的噬菌体进行了研究，从阿里亚克海的沉积物中分离到4个噬菌体。所有分离的噬菌体仅能感染创伤弧菌NBRC 15645^T，导致宿主特异性很小。电子显微镜分析表明，噬菌体1号的大小为200 nm×800 nm。还测定了创伤弧菌NBRC 15645℃下分离的噬菌体的裂解曲线。此外，还从分离的噬菌体中进行了基因组提取，但产率相当低。另一方面，将创伤弧菌ATCC27562~T株或创伤弧菌K-6株分别皮下注射于C57BL/J6小鼠后腿底部，成功地建立了创伤弧菌和创伤弧菌的动物模型。此外，我们还对6只C57BL/J6小鼠的后腿底部皮下注射K-6株或L-K-6株，用噬菌体裂解后的K-6株或L-K-6株。结果，注射K-6毒株6只小鼠中有4只死亡，而注射L-K-6毒株无一只死亡。此外，与K-6菌株相比，L-K-6菌株的生长速度降低，溶血活性增强。

A bacteriophage which infects Vibrio vulnificus was searched, and four bacteriophages were isolated from the sediments in Ariake Sea. All isolated bacteriophages could infect only V.vulnificus NBRC 15645^T, which resulted in a quite narrow host specificity. Electron microscopical analysis revealed that the size of bacteriophage No.1 was 200 nm×800 nm. The lysis curve of isolated bacteriophages infecting V.vulnificus NBRC 15645^T were also investigated. Furthermore, a genome extraction from isolated bacteriophages was performed, but the yields were quite low. On the other hand, we gave a hypodermic injection of V.vulnificus ATCC27562^T strain or V. vulnificus K-6 strain to C57BL/J6 mouse's hind leg at the bottom, which succeeded in animal model infected with both V.vulnificus strains. In addition, we also gave a hypodermic injection of K-6 strain or L-K-6 strain, which was lysogenized with its bacteriophage, to six C57BL/J6 mice's hind leg at the bottom. As a result, the injection of K-6 strain killed four of six mice while no mice were killed by L-K-6 strain. Furthermore, L-K-6 strain decreased the growth rate and increase hemolytic activity, compared with K-6 strain.