Development of analysis system of gene expression cascade using in vitro virus

由于顺式调控DNA元件和转录因子之间的特异性相互作用是转录调控网络的关键组成部分，因此DNA-转录因子相互作用的全面分析有望提供对细胞增殖、组织形态发生中的发育过程和疾病机制的深入理解。在这项研究计划中，我首次表明，体外病毒（IVV）选择系统，mRNA展示技术，可用于阐明新的，具有生物学意义的DNA-蛋白质相互作用。本文所述的IVV系统将促进DNA-转录因子相互作用的大规模分析，以在全基因组范围内绘制转录调控网络。

Since the specific interactions between cis-regulatory DNA elements and transcription factors are critical components of transcriptional regulatory networks, comprehensive analysis of DNA-transcription factor interactions is expected to provide a deep understanding of the mechanisms of cell proliferation, developmental processes in tissue morphogenesis, and disease. In this research program, I have shown for the first time that in vitro virus (IVV) selection system, an mRNA display technique, can be employed for the elucidation of new, biologically significant DNA-protein interactions. The IVV system described herein will facilitate the large-scale analysis of DNA-transcription factor interactions for mapping of transcriptional regulatory networks on a genome-wide scale.