Use of Biosurfactants for Monitoring and Remediation of Persistent Organic Pollutants

从哺乳动物生物学功能的分子机制出发，试图开发方便的多氯联苯。(1)将重组芳烃受体（AhR）介导的β-葡萄糖醛酸酶（GUS）报告基因表达体系分别引入烟草和拟南芥的生化检测中。转基因植株用于多氯联苯GUS检测。特别是，生物表面活性剂的使用提高了转基因植物中多氯联苯的GUS检测灵敏度。(2)制备多氯联苯特异性单克隆和重组抗体，建立多氯联苯酶联免疫吸附法（elisa）。elisa选择性检测共面多氯联苯。

It was attempted to develop convenien polychlorinated biphenyls(PCBs) based on molecular mechanisms of biological functions in mammals. (1) Recombinant aryl hydrocarbon receptor(AhR)-mediatedβ-glucuronidase(GUS) reporter gene expression systems were each introduced into tobacco t biochemical assays of and Arabidopsis plants. The transgenic plants were developed for GUS assays of PCBs. Particularly, the use of biosurfactants improved GUS assay sensitivity of PCBs in the transgenic plants. (2) Monoclonal and recombinant antibodies specific to PCBs were prepared for development of Enzyme-Linked Immunosorbent Assays(ELISAs) of PCBs. The ELISAs selectively detected coplanar PCBs.