Relationship between the common epitope and the structure of sugar chains in plant allergens

我们研究了大豆过敏原Gly m Bd 28 K和小麦过敏原Tri a Bd 27 K的致敏性。这些过敏原除甘露糖、N-乙酰葡糖胺外还含有岩藻糖和木糖，表明这些过敏原的糖部分可能是天冬酰胺连接的糖链。此外，这些糖部分被证明是结合IgE抗体在患者血清中的敏感大豆和小麦。Gly m Bd 28 K的cDNA已被克隆。然而，该cDNA具有编码Gly m Bd 28 K前体的信号肽的部分，但该cDNA不具有编码过敏原的全长mRNA的完美cDNA。在本研究中，信号肽上的三个氨基酸残基和5 '-非翻译区的20-bp的5'-RACE试剂盒被解析。这一发现提供了关于过敏原在培养的烟草细胞中表达的有用信息。

We investigated the allergenicity of soybean allergen, Gly m Bd 28K, and wheat allergen, Tri a Bd 27K. These allergens were shown to contain fucose and xylose in addition to mannose, N-acetyl glucosamine, indicating that the sugar moieties of these allergens may be asparagine-linmked sugar chain. Furthermore, these sugar moieties were shown to be bind IgE antibodies in sera of patients sensitive to soybean and wheat.The cDNA encoding Gly m Bd 28K has been cloned. However, the cDNA has the part encoding the signal peptide of the precursor for Gly m Bd 28K, but the cDNA has not the perfect cDNA encoding full length of mRNA for the allergen. In the present study, three amino acid residues on the signal peputide and 20-bp on the 5'-untranlated region were elucidated by 5'-RACE kit. This finding provide useful information on the expression of the allergen in cultivated tabacco cells.