Development of a dual-imaging sentinel lymph node detection method with the use of fluorescent liposome system

设计了一种新型荧光脂质体系统，旨在延长前哨淋巴结（SNs）的保留时间，同时体外检测SNs的可用性。用引入烷基链的ICG衍生物、磷脂和油性放射造影剂构建脂质体，并在小鼠模型中进行检测。注射后5小时，这些药物立即积聚到原发性淋巴结，没有进一步扩散。然而，由于脂质体内造影剂的最大含量较低，x线计算机断层扫描（CT）难以检测到堆积。因此，这些脂质体被制成不模糊的组织标记物，与聚甘油-聚蓖麻油酸共聚物聚集形成巨大的囊泡簇。应用红外荧光成像和x线CT三维重建图像对该标记物在家猪胃壁内的扩散进行扫描。该标记物可实现腹腔镜早期胃肠道肿瘤切除术的术前模拟和术中导航。

A novel fluorescent liposome system was designed to aim at longer retention time in sentinel lymph nodes (SNs) and availability of extracorporeal detection of SNs at the same time. Liposomes were constructed with ICG derivative in which an alkyl chain was introduced, phospholipids and oily radiographic contrast medium, and tested in mouse models. Those showed immediate accumulation into primary lymph nodes and no further diffusion even five hours after injection. However, it was difficult to detect the accumulation with X-ray computed tomograph (CT), because of low maximum content of the contrast medium within the liposome. Thus, those liposomes were made into non-blurring tissue marker, aggregating with polyglycerol-polyricinoleate copolymer to make into giant vesicle clusters. Diffusion of this marker 18 hours after administered into gastric wall of domestic pig was scantling with infrared fluorescent imaging and 3D reconstructed image of X-ray CT. This marker could contribute to realize preoperative simulation and intraoperative navigation for laparoscopic resection of early gastrointestinal cancer with a single administration.