FGF signaling accelerate epithelial regeneration by Oral Epithelial Stem Cells

成纤维细胞生长因子-10(成纤维细胞生长因子-10)是一种已知的上皮细胞有丝分裂原，存在于多种器官系统，如肺，以双向旁分泌的方式发挥作用，并仅通过FGFR2b等受体的子集发挥作用。然而，它在口腔上皮再生中的作用尚不清楚。我们利用FGFR2b过表达(OE)和FGFR2b衰减/敲除(KD)转基因小鼠，研究了FGFR2b/FGFR10信号在口腔上皮损伤修复中的作用。结果提示：1)FGFR2b KD小鼠上皮层发育不良，而FGFR2b-OE小鼠上皮层发育过度。2)FGF10过表达导致口腔上皮或尿路上皮角化不全；3)FGF10过表达促进复层鳞状上皮和移行上皮基底层的增殖。从这些结果来看，FGFR2b/FGFR2b信号在口腔上皮干细胞中起活跃的增殖作用，并加速口腔上皮细胞的再生能力。

Fibroblast growth factor-10(FGF-10) is a known epithelial mitogen in several organ systems, such as the lung, that acts in a bidirectional paracrine manner and acts exclusively through a subset of receptors such as FGFr2b. However, its role in oral epithelial regeneration is unknown. We investigated the role of FGFr2b/FGF-10 signaling in oral epithelial wound healing using FGF-10 overexpression(OE) and FGFr2b attenuation/knock-down(KD) transgenic mice. The present results suggested that, 1) Under-developing of the epithelial layer was noted in the FGFr2b KD mice, whereas over-developing was noted in the FGF-10 OE mice. 2) FGF10 overexpression initiated the parakeratosis of oral epithelium or urothelium and 3) FGF10 overexpression worked the proliferation of the basal laminae in the stratified squamous epithelium and transitional epithelium. Form these results, FGF-10/FGFr2b signaling appears to play an active proliferation in Oral Epithelial Stem Cells, and accelerate the regenerative capacity of the oral epithelium.