A novel approach to treat severe trauma using gene introduction by nanomicelle into skeletal muscle

复合纳米胶束是一种有效的、具有生物相容性的基因载体。通过水动力注射纳米胶束进入小鼠骨骼肌，获得了一个多月的长时间转基因表达。在纳米胶束中添加硫酸软骨素进一步减轻了肌肉组织损伤。在周围神经损伤模型小鼠中，利用纳米胶束将IGF-1基因导入肌肉，通过IGF-1诱导的肌肉肥大过程，加速了瘫痪肢体运动功能和感觉的恢复。这种策略有望避免废用性萎缩，并最终在严重创伤后早期恢复运动和感觉功能。

Polyplex nanomicelle is an effective and biocompatible gene carrier. By hydrodynamic injection of nanomicelle into skeletal muscle of mice, a prolonged transgene expression was obtained for more than a month. The addition of chondroitin sulfate to the nanomicelle further reduced the tissue damage in the muscle. In model mice of peripheral nerve injury, the introduction of IGF-1 gene into the muscle using the nanomicelle accelerated the recovery of motor function and sensation of the paralyzed limb, through the processes of the muscular hypertrophy induced by IGF-1. This strategy is promising for avoiding disuse atrophy and the eventual early recovery of motor and sensory functions after severe trauma.