Generation of knockout mice and transgenic mice of the Alivin family

为了研究Alivin家族的生物学功能，我们产生了携带Alivin1敲除（KO）等位基因、Alivin2 KO等位基因、Alivin3 KO等位基因和Alivin1过表达转基因基因的小鼠突变株。野外实验结果表明，过表达ALIVIN1转基因小鼠的焦虑感增强。与野生型相比，从Alivin1 KO小鼠胚胎制备的成纤维细胞的迁移活性降低。

In order to study biological functions of the Alivin family, we have generated mutant stains of mice harboring Alivin1 knockout (KO) allele, Alivin2 KO allele, Alivin3 KO allele, and an Alivin1 over-expressing transgene. Results of open-field tests suggest that the transgenic mice over-expressing ALIVIN1 have enhanced anxiety. The fibroblast prepared from embryos of the Alivin1 KO mice showed reduced migratory activity compared with that of wild type.