Mechanism of cutaneous vasodilation following acupuncture stimulation-Investigation using microdialysis-

我们旨在通过研究一氧化氮（NO）和轴突反射性血管舒张的作用来阐明针刺刺激后皮肤血管舒张的机制。通过比较利多卡因乳膏治疗部位和未治疗部位，探讨感觉神经元轴突反射性血管舒张的作用。尽管使用利多卡因乳膏治疗的部位机械刺激的感觉阈值增加，但随着时间的推移，治疗部位和未治疗部位的皮肤血管传导（CVC）和面积的变化没有显著差异。然后，我们通过皮内微透析，皮内给予NO合成酶抑制剂N^ g -硝基- l -精氨酸甲酯盐酸盐（L-NAME 20 mM）来研究NO的作用。与对照组（给予林格溶液）相比，给予L-NAME的部位在针刺刺激后的一段时间内CVC显著降低。这些发现提示针刺刺激后皮肤血管扩张的机制不包括感觉神经元的轴突反射性血管扩张，且一氧化氮部分参与。

We aimed to elucidate the mechanism of cutaneous vasodilation following acupuncture stimulation by investigating the roles of nitrogen monoxide (NO) and axon reflex vasodilation. The role of axon reflex vasodilation by sensory neurons was investigated by comparing sites treated with lidocaine cream with untreated sites. Although the sensory threshold for mechanical stimulation increased in sites treated with lidocaine cream, changes over time in cutaneous vascular conductance (CVC) and area did not significantly differ between treated and untreated sites. We then investigated the role of NO by intradermally administering N^G-nitro-L-arginine methyl ester hydrochloride (L-NAME 20 mM), an NO synthase inhibitor, using intradermal microdialysis. Compared to controls (administered Ringer's solution), sites administered L-NAME showed a significant reduction in CVC over time following acupuncture stimulation. These findings suggest that mechanisms other than axon reflex vasodilation by sensory neurons are involved in cutaneous vasodilation following acupuncture stimulation, and that NO is partially involved.