Regeneration of alveolar bone using RNAi via gastrointestinal hormone 'Incretin' signal pathway.

胰岛素是胃肠激素家族中的一员，在消化和摄取食物时由肠道导管分泌，通过促进胰腺分泌胰岛素来控制血糖水平。近年来，有报道称胰岛素可能会影响骨代谢。因此，本研究的目的是探讨胰岛素促进牙槽骨再生的能力。首先，我们用MC3T3-E1分析了细胞表面受体(GIP-R、GLP-1-R、Ob-Rb)的表达，但结果不稳定。另一方面，围绕糖与钙代谢的关系，我们利用糖尿病动物模型(STZ诱导的糖尿病大鼠、Wistar肥胖大鼠)分析了胰岛素信号对骨代谢的影响。我们发现胰岛素耐量不足会导致颅面部严重的生长迟缓，特别是颅骨、下颌骨和牙槽骨等硬组织。我们还发现，幼年阶段胰岛素缺乏导致了大鼠牙齿形成的紊乱。

Incretin, a family of the gastrointestinal hormone, which is secreted from intestinal ducts upon the digestion and intake of food, and controls the blood glucose level by promoting insulin secretion from pancreas. Recently, it has been reported that incretin might affect on bone metabolism. So the purpose of this research was to explore the ability of incretin to promote alveolar bone regeneration. At first, we analyzed the receptor expression of cell surface (GIP-R, GLP-1-R, Ob-Rb) using MC3T3-E1, but the result of receptor expression were unstable. On the other hand, focusing on the relationship between glucose and calcium metabolism, we analyzed the insulin signal effects on bone metabolism using diabetes animal models (STZ-induced diabetic rat, Wistar fatty rat). We found that poor insulin tolerance result in the severe growth retardation in craniofacial region, especially hard tissue like cranium, mandible and alveolar bone. And we also found that insulin deficiency from infant stage caused the disorder of tooth formation in rats.