Modulation and optimization of immune response utilizing complement regulatory factors in fish

为了阐明补体调节因子Tecrem和备解素（Pf）调节鱼类免疫应答的分子机制，对斑马鱼、鲤鱼和银鲫的Tecrem进行了基因多样性和mRNA及蛋白质水平的功能分析。本研究成功制备了重组鲤鱼Tecrem，并建立了抗鲤鱼Tecrem的单克隆抗体，阐明了Tecrem在组织和细胞中的广泛分布，以及对病毒感染的表达反应，提示Tecrem在宿主免疫系统中的重要作用

In order to elucidate molecular mechanism of complement regulatory factors, Tecrem and properdin (Pf), in modulating immune responses in fish, gene diversity, functional analysis at both mRNA and protein levels of zebrafish, carp, and ginbuna crucian carp Tecrem were conducted. We succeeded to prepare of recombinant carp Tecrem and to establish monoclonal antibody to carp Tecrem, and elucidated its wide tissue and cellular distribution, expression response to viral infection, suggesting key role of Tecrem in host immune system