Molecular characterization of sensitized house dust mite allergens for the generation of tailor-made vaccine

过敏原特异性免疫疗法（SIT）是唯一有希望治疗过敏性疾病的方法，但目前的SIT存在副作用风险等问题。本研究旨在构建重组屋尘螨变应原文库，并将其应用于屋尘螨的分子诊断和免疫治疗。在这里，我们成功和全面地生产了23个主要的屋尘螨过敏原作为功能重组蛋白。通过使用这些重组过敏原分子，我们还开发了一种分子诊断系统，使我们能够确定螨虫过敏个体中的致敏过敏原。

Allergen-specific immunotherapy (SIT) is the only promising treatment of allergic disorders, but current SIT has problems such as the risk of side effects. Objective of this study is to construct a recombinant house dust mite allergen library, and to apply those allergen molecules for the development of molecular diagnostics and immunotherapeutics. Here, we showed a successful and comprehensive production of 23 major house dust mite allergens as a functional recombinant proteins. By using those recombinant allergen molecules, we also developed a molecular diagnostic system that enabled us to determine sensitized allergens among mite-allergic individuals.