Molecular mechanisms for NOSs-mediated responses in microvessels

我们证明，骨髓（BM）在调节微血管内皮和代谢功能中起着重要作用。我们使用雄性eNOS（-/-）小鼠，其移植有来自野生型（WT）或eNOS（-/-）小鼠的BM细胞。我们证明，内皮依赖性舒张和肠系膜动脉乙酰胆碱的超极化降低eNOS（-/-）小鼠，并显着改善移植WT-BM时，而不是eNOS（-/-）-BM。过氧化氢酶可消除内皮依赖性舒张反应的增强成分，表明H_2O_2介导了内皮依赖性舒张反应的增强。相比之下，在主动脉中没有观察到这种有益作用。

We demonstrated that bone marrow (BM) plays an important role in modulating microvascular endothelial and metabolic functions. We used male eNOS(-/-) mice which were transplanted with BM cells from wild-type (WT) or eNOS(-/-) mice. We demonstrated that endothelium-dependent relaxations and hyperpolarizations of mesenteric arteries to acetylcholine were reduced in eNOS(-/-) mice and were markedly improved when transplanted with WT-BM but not with eNOS(-/-)-BM. The enhanced component of endothelium-dependent relaxations was abolished by catalase, indicating that the improved responses were mediated by H_2O_2. In contrast, no such beneficial effect was noted in the aorta.