Investigation the mechanisms on invasion and metastasisof oral squamous cell carcinoma

我们一直在研究肿瘤的侵袭方式作为口腔鳞癌患者的重要预后因素。Jakobson提出的侵袭方式4级可分为4C级(索状型)和4D级(弥漫型)。这项研究的目的是用免疫组织化学和实验的方法阐明这种改良的侵袭模式分级系统。材料和方法采用OCS-20(Gr.3)、OSC-19(Gr.3)、OSC-19(Gr.3)三种细胞系建立体外和体内侵袭模型。4C)和HOC-313(Gr.4D)。在免疫组织化学染色的人癌组织中，侵袭方式分级越高，4型胶原的连续性越差，E-钙粘附素的表达越少。大多数弥漫性浸润性癌症(Gr.4C和4D)表达MMPs和HIF-1。在细胞侵袭实验中，HOC-313表现出最大的运动能力(AMF)。在3T3成纤维细胞胶原凝胶上培养的细胞株表现出与其来源相似的侵袭模式。其中，即使未包埋成纤维细胞，也只有HOC-313细胞具有侵袭性。OSC-20和OSC-19裸鼠舌内原位移植生长并转移至淋巴结。它们的组织学形态与来源相似。在Matrigel的支持下，HOC-313暂时生长了几个月。在最近的研究中，OLC-01(Gr.4D)细胞在裸鼠体内产生了致命的致瘤性，其组织学与来源相似。

We have been studying the mode of cancer invasion as the important prognostic factor in patients with oral squamous cell cancer. Grade 4 of mode of invasion proposed by Jakobsson was subclassified into grades 4C (Cord-like type) and 4D (Diffuse type). The purpose of this study is to immunohistochemically and experimentally elucidate this modified grading system for the mode of invasion. Materials and methods used are human cancer materials and in vitro and in vivo invasion model by using 3 cell lines; OCS-20 (Gr.3), OSC-19 (Gr. 4C) and HOC-313 (Gr. 4D). In immunostained human cancer materials, the higher the grade of the mode of invasion was, the less continuous the type 4 collagen was, and the less E-cadherin was. Majority of diffuse invasive cancers (Gr. 4C and 4D) expressed MMPs and HIF-1. In cell invasion assay, HOC-313 showed the greatestmotility (AMF). Cell lines cultured on 3T3 fibroblast-embedded collagen gels showed similar invasion pattern to their origin. Among them, only HOC-313 showed invasion evenif fibroblast was not embedded. OSC-20 and OSC-19 implanted orthotopically in the tongue of nude mice grew and metastasized to lymph nodes. Their histologic appearance was similar to that of the origin. HOC-313 grew temporarily for several months by support of matrigel. In recent study, cell selected OLC-01 (Gr.4D) produced lethal tumorgenicity in nude mouse with similar histology to the origin.