Organelle imaging of signal transduction towards necrotic cell death

为了研究细胞内信号转导机制，我们测量了亲环素D对线粒体能量代谢的影响以及诱导坏死后观察到的线粒体变化。通过光学显微镜观察单个线粒体的变化。结果，我们成功地1）检测亲环素D对细胞中丙酮酸代谢的影响，2）检测细胞中持久的线粒体渗透性转变，和3）线粒体渗透性转变和线粒体肿胀的同时成像。这些结果提供了重要的信息坏死的机制作为程序性细胞死亡。

In order to investigate the mechanism of intracellular signal transduction towards necrosis, we measured the effects of cyclophilin D on the energy metabolism of mitochondria and the changes of mitochondria observed upon induction of necrosis. The changes were detected by imaging single mitochondria with optical microscopy. As a result, we succeeded in 1) detection of the effects of cyclophilin D on the pyruvate metabolism in cells, 2) detection of long-lasting mitochondrial permeability transition in cells, and 3) simultaneous imaging of mitochondrial permeability transition and mitochondrial swelling. These results provide important information on the mechanism of necrosis as programmed cell death.