Development of a new probe of a flavonoid metabolite, DOPAC, for understanding the biomolecule modification

人们对大肠发酵产物的兴趣已经开始迅速增长，例如一种栎素葡萄糖苷代谢物3，4-二羟基苯乙酸(DOPAC)。在这项研究中，我们通过了解与其化学特性相关的生物活性的分子机制，确定了几个可能的多酚目标分子。我们还发现，DOPAC具有更强的清除自由基和II期药物代谢酶的活性，比其他槲皮素代谢产物更有效。此外，我们还开发了一种新的探针(DOPAC炔丙基酯)来检测目标蛋白，并证实了它在“点击化学”中的适用性。

The interest in fermented products in the large intestine such as a quercetin glucoside metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), has started to grow rapidly. In this study, we identified several plausible target molecules for polyphenols by understanding the molecular mechanisms underlying biological activities related to their chemical characteristics. We also revealed that DOPAC exhibited radical scavenging and phase II drug metabolizing enzyme-inducing activities more potently than other quercetin metabolites. Furthermore, we developed a new probe (DOPAC propargyl ester) to detect target proteins and confirmed its applicability to “click chemistry”.