Studies on anti-allergic properties of a mixture of Saccaromyces pastorianus and its specific cow's milk antibody

作者制备了一种富含免疫球蛋白的组份，该组份是Saccarmyces(S.)巴氏杆菌(牛奶抗体)，来自经巴氏杆菌免疫的奶牛产生的初乳。添加巴氏葡萄球菌和牛奶抗体混合饲料的小鼠的鼻部摩擦反应和BALB/c小鼠的耳厚度显著低于添加巴氏葡萄球菌和牛奶抗体饲料的小鼠(对照组)。添加混合饲料的NC/NGA小鼠的Fc、ε、RI、α+CD117+细胞数和STIM1mRNA的表达均显着低于对照组。在加入巴氏葡萄球菌和牛奶抗体混合培养的小鼠脾细胞中，Ig E受体各亚单位链FcεRIα、FcεRIβ和FcεRIγ以及与脱颗粒有关的蛋白如STIM1、BTK和ITKmRNA的表达均显著低于不加巴氏葡萄球菌和/或牛奶抗体的小鼠脾细胞。另一方面，在给予每克体重2000毫克巴氏葡萄球菌和/或牛奶抗体的ICR小鼠中，仅观察到轻微的急性毒性。这些结果表明，巴氏葡萄球菌与牛奶抗体的混合物是一种安全的抗过敏食品材料。

The author prepared an IgG-rich fraction specific to Saccaromyces (S.) pastorianus (milk antibody) from colostrum produced by a S. pastorianus-immunized cow. The allergic symptoms indicated as nose rubbing events of miceNC/Nga mice and ear thickness of BALB/c mice given a diet containing amixture of S. pastorianus and milk antibody (mixture-added diet) were significantly lower than in those given the S. pastorianus and milk antibody-free diet (control diet). The number of FcεRIα+CD117+ cells and mRNA expression of STIM1 were significantly lower in spleens of NC/Nga mice given the mixture-added diet than in those given the control diet. The mRNA expression of all subunit chains of IgE receptor, i.e. FcεRIα, FcεRIβ and FcεRIγ and proteins such as STIM1, BTK and ITK relating to degranulation was significantly reduced in C3H/HeN mouse spleen cell cultures with a mixture of S.pastorianus and milk antibody than in those without the S. pastorianus and/or milk antibody. On the other hand, only minimal acute toxicity was observed in ICR mice given 2,000 mg of S. pastorianusand/or milk antibody/g body weight. These results suggest that the mixture of S. pastorianus and milk antibody represents a safety anti-allergic food material.