Analysis of replication initiation systems regulated through the proteins-interacting domain of DnaA

染色体复制的起始是严格控制的，以确保染色体DNA在所有细胞生物中每个细胞周期只复制一次。在大肠杆菌中，dna启动器失活以抑制dna复制酶夹紧复合物的额外启动。本研究表明，dna Nand c端结构域的特定氨基酸残基对于与Hda的相互作用很重要。此外，通过蛋白质纯化方法寻找新的起始调节因子候选物，然后通过质谱分析鉴定。

The initiation of chromosomal replication is strictly controlled to ensure that chromosomal DNA is replicated only once per cell cycle in all cellular organisms. In Escherichia coli, the DnaA initiator is inactivated to repress extra initiations by the Hda-replicase clamp complex. Here, this study showed that specific amino acid residues of DnaA Nand C-terminal domains are important for interaction with Hda. Moreover, candidates of novel initiation regulation factors were searched by protein purification methods, and then identified by mass spectrometry analyses.