Induction of Jaw Bone and Tooth Germ from murine embryonic stem cells and human bone marrow stem cells in serum-free defined suspension culture

人胚胎干细胞(HES)和人诱导多能干细胞(HIPS)通常以灭活的小鼠胚胎成纤维细胞为饲养细胞，在添加胎牛血清或专有替代物的培养液中维持。由于相对缺乏关于细胞对分化生长因子的反应的知识，含有未定义或未知成分的培养液的使用限制了多能细胞应用的发展。因此，我们开发了一种无血清培养液，命名为hESF9，在这种无血清培养液中，人ES细胞可以在没有饲养细胞的情况下保持未分化状态。我们开发了一种无血清限定的培养HIPS细胞的培养液，它可以保持增殖、自我更新和多能性。由于这种简单的无血清贴壁单一培养体系将使我们能够在特定的条件下阐明细胞对生长因子的反应，并可以消除未知病原体可能带来的风险。

Human Embryonic Stem(hES) cells and human induced Pluripotent Stem(hiPS) cells are commonly maintained on inactivated mouse embryonic fibroblast as feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture media containing undefined or unknown components has limited the development of applications for pluripotent cells because of the relative lack of knowledge regarding cell responses to differentiating growth factors. Therefore we developed a serum-free medium, designated hESF9, in which human ES cells can be maintained in undifferentiated state without feeder cells. We have developed a serum-free defined medium for culturing hiPS cells, which can maintain proliferation, self-renewal and pluripotency. As this simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions, and can eliminate the risk might be brought by undefined pathogens.