Sophistication of supported molecular matrix electrophoresis (SMME) for highly glycosylated proteins "mucins"

支持分子基质电泳法(SMME)是近年来由主要研究者发展起来的一种用于粘蛋白分析的新型膜电泳法。为了利用SMME进行粘蛋白生物标记物的发现研究，SMME已经通过特别努力提高分离的分辨率和粘蛋白的灵敏检测而得到完善。该成果包括一种良好的SMME膜的制备方法，良好分离粘蛋白的电泳法条件，一种新的染色方法“琥珀酰化-阿新蓝”，一种使SMME膜透明的方法，一种带有抗串联重复抗体的粘蛋白染色方法，以及一种凝集素亲和力-SMME。

Supported molecular matrix electrophoresis (SMME) is a novel membrane electrophoresis which has been recently developed for mucin analysis by the principal investigator. To utilize the SMME for mucin biomarker discovery research, sophistication of SMME has been performed by making a special effort to improve both the resolution of separation and the sensitive detection of mucins. The achievement includes a preparation method for a good SMME membrane, electrophoresis condition for good separation of mucins, a novel staining method "succinylation-Alcian blue", a procedure for making the SMME membrane transparent, a staining method for mucins with anti-tandem repeat antibodies, and a lectin affinity-SMME.