Escherichia coli host engineering for non-biased expression of metagenomic library

我们应用翻译工程的方法解决了异源表达问题。启动因子3和核糖体被设计来改变翻译谱，并发现了具有非AUG起始密码子的基因表达增强的变体。此外，突变的核糖体具有来自非E。大肠杆菌表现出报告GFP基因的改变或增强表达，而这些基因在野生型大肠杆菌中不能有效表达。

We have applied translational engineering approach to solve the problem in heterologous expression problem in Escherichia coli. Initiation factor 3 as well as the ribosome was engineered to alter the translational profile and variants that showed enhanced expression of genes having non-AUG initiation codon were identified. Also, mutant ribosome that have 16S rRNA from non-E. coli bacteria showed altered, or enhanced expression of reporter gfp genes, that were not efficiently expressed in wild type E. coli.