Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis

为了分离参与异黄酮生物合成的转录因子，我们利用新一代测序仪器illumina HiSeq 1000在德国鸢尾根培养物中建立了EST（表达序列标签）数据，积累了异黄酮。此外，我们在德国虹膜根培养细胞传代过程实验中进行转录表达分析（使用定制的虹膜DNA阵列），用于基因共表达网络分析中的基因发现。我们建立了独立的Confeito Java-GUI（基因共表达网络分析）软件，用于提取高度互联的基因模块。利用公开的拟南芥DNA阵列资源数据（超过9000个），我们展示了我们开发的Confeito Java-GUI软件在拟南芥脂肪族硫代葡萄糖苷生物合成中基因共表达网络模块的应用。

In order to isolate transcription factors involved in isoflavonoid biosynthesis, we established an EST (expressed sequence tag) data in Iris germanica root cultures, accumulating isoflavonoid, using next-generation sequencing instrument illumina HiSeq 1000 with 100 bp paired-end reads. Furthermore, we carrying out transcript expression analysis (using a custom Iris DNA array) in subculturing time-course experiment of Iris germanica root cultured cells for gene discovery in gene co-expression network analysis.We established standalone Confeito Java-GUI (gene co-expression network analysis) software that extracted highly-interconnected gene modules. Using the public Arabidopsis DNA array resource data (more than 9 thousands), we have shown an application of our developed Confeito Java-GUI software to isolate gene co-expression network module in Arabidopsis aliphatic glucosinolate biosynthesis.