Sialyllactose - modified 3-way junction DNA as a inhibitor of influenza hemagglutinin

唾液乳糖（Sialyllactose， SL）存在于细胞表面，在流感病毒感染中起重要作用。SL被病毒上的血凝素（HA）蛋白识别。一种抑制细胞上SL与血凝素相互作用的化合物被认为可以预防流感病毒感染，因为has中唾液酸结合位点的氨基酸序列在流感病毒株中高度保守。在本研究中，合成了sl修饰的dUTP作为DNA聚合酶的底物，并将这些sl结合在3-way junction DNA上。这些dna与流感病毒A/PR/8/34（H1N1）的血凝素具有高亲和力（15 nM）。这些dna有望被用作流感病毒感测或流感感染抑制剂。

Sialyllactose(SL) is present on cellular surface and plays a important roles in the infection on influenza virus. The SL is recognized by hemagglutinin(HA) protein on the virus. A compound that inhibits the interaction between SL on the cell and hemagglutinin has been thought to prevent infection of influenza virus, because the amino acids sequence of sialic acid binding site in HAs are highly conserved among influenza virus strains. In this study, SL-modified dUTP was synthesized as a substrate of DNA polymerase, and these SLs were incorporated on 3-way junction DNAs. These DNAs have high affinity (15 nM) to the hemagglutinin on influenza virus A/PR/8/34(H1N1).These DNAs are expected to be used as influenza virus sensing or inhibitors of influenza infection.