Enhancement by liposome of semi-synthesis of specifically modified protein at the C-terminal and application to living cell

在我们之前的研究中，曾进行过亲脂化合物修饰蛋白质的半合成，但效果不佳。在特定缩合过程中的变性蛋白、偶联产物的纯化以及对活细胞的毒性都需要进行有效的改进。为了克服这些困难，在每一步中都使用了脂质体。结果表明，从缩合反应混合物中分离偶联产物，避免蛋白质变性的缩合反应顺利进行。然而，在不使用洗涤剂的情况下，使用脂质体进行偶联并不有效。此外，当脂质体荷尔蒙修饰蛋白应用于活细胞时，不能有效地从脂质体中释放蛋白质。

In our previous study, semi-synthesis of protein specifically modified with lipo-philic compounds was carry out, but not effective. Denaturing protein during the specific condensation, purification of coupling product, and toxicity to live cell are to be adapted for improving effectively. To overcome these difficulties, liposome was employed in each step. As results, separation of coupling product from reaction mixture of condensation, and condensation avoiding denature of protein were carried out smoothly. However, use of liposome in coupling without detergent was not effective. Andmore, release of protein from the liposeme was not effective when liposeme horboring modified protein was applied to living cell.