What mechanisms determine the final destination of GPI-anchored proteins?

在真菌中，一些gpi锚定蛋白（GPI-APs）保留在质膜上，而另一些则转移到细胞壁上。我们研究了酿酒酵母cwh43缺失突变细胞细胞壁和质膜中GPI-AP的含量，其中GPI-AP的脂质部分没有转化为神经酰胺类型。在cwh43突变细胞中，细胞壁中GPI- ap的数量增加，表明GPI脂质部分向神经酰胺的转化（脂质重塑）有助于GPI- ap保留在质膜上。在ted1缺失突变细胞中，GPI的聚糖结构发生改变，质膜中GPI- ap的数量增加，表明GPI的聚糖结构影响GPI的脂质重塑。

In fungi, a number of GPI-anchored proteins (GPI-APs) are retained to the plasma membrane, whereas others are transferred to the cell wall. We investigated amounts of GPI-AP in the cell wall and plasma membrane in Saccharomyces cerevisiae cwh43 deletion mutant cells, in which lipid moieties of GPI-APs are not converted to ceramide types. In cwh43 mutant cells, the amount of GPI-AP in the cell wall is increased, indicating that the conversion (lipid remodeling) to ceramides in GPI lipid moieties contributes to the retention of GPI-APs to the plasma membrane. In ted1 deletion mutant cells, in which structure of glycans of GPI is altered, the amount of GPI-AP in the plasma membrane is increased, indicating that glycan structures of GPI influences the lipid remodeling of GPI.