Identification of novel genes for retinoic acid receptors and its function in endometrial cancer.

我们重点研究了RARa和ERa互转录因子的新靶区。然后，我们试图检测这些基因及其新的分子机制。首先，我们揭示了RARa和ERa之间的相反功能。然后，我们利用ChIP克隆技术研究了RARa和ERa的靶结合区，发现了SMAD3的启动子病变。此外，与对照数据相比，敲除RARa和添加AM580处理后SMAD3显著降低。我们的研究结果表明，与AM580治疗密切相关的SMAD3基因可能在子宫内膜癌中发挥重要的生物学作用。

We focused on the novel target areas of mutual transcription factor for RARa and ERa. And then, we tried to detect the genes and its novel molecular mechanism.At first, we revealed the opposite functions between RARa and ERa. And then, we studied the target binding area of RARa and ERa using ChIP cloning technique, and found the promoter lesions of SMAD3. In addition, SMAD3 was significantly decreased after knock-down for RARa and addition of AM580 treatment compared with the control data.Our findings suggested that SMAD3 genes, which were deeply correlated with AM580 treatment, might play important biological roles in endometrial cancer.