Real-time monitoring of Ca2+ responses in rat submandibular glands and salivary secretions in living animals.

为了研究活体动物唾液分泌与Ca ~（2+）反应之间的关系，我们实时测量了大鼠下颌下腺（SMG）的Ca ~（2+）反应和SMG的唾液分泌。首先，我们测量了促涎剂诱导的Ca 2+反应和唾液分泌。施用各种激动剂诱导整个Ca 2+传感器表达的SMG的细胞内Ca 2+浓度（[Ca 2 +]i）增加。此外，乙酰胆碱在整个腺体中经常显示出Ca 2+振荡。我们发现，在活体动物中，激动剂引起的[Ca ~（2+）]i的时空变化存在差异。当使用微压力传感器测量唾液分泌物与Ca 2+响应时，分泌物发生与[Ca 2 +]i的增加的延迟。Stim 1基因的表达增强了激动剂诱导的SMG细胞内钙离子反应，是激活钙离子内流的关键基因，因此，Stim 1基因的表达可能是未来口干症基因治疗的一个理想策略。

To investigate the relationships between Ca2+ responses and salivary secretion in living animals, we measured Ca2+ responses in the rat submandibular gland (SMG) and salivary secretion from the SMG in real-time. First, we measured sialogogue-induced Ca2+ responses and salivary secretions. Administration of various agonist induced increase in intracellular Ca2+ concentration ([Ca2+]i) throughout the Ca2+ sensor-expressed SMG. In addition, acetylcholine frequently shows Ca2+ oscillations in whole gland. We found that there is the difference among the agonist-induced spatio-temporal changes in [Ca2+]i in living animals. When salivary secretions were measured with Ca2+ responses using the micro pressure sensor, secretions occurred with a delay from the increase in the [Ca2+]i. The expression of Stim1, which is essential for the activation of Ca2+ entry, increased agonist-induced Ca2+ responses in SMG cells, therefore it may be a suitable gene therapy strategy for dry mouth in the future.