Attempts to identify the hard tissue regeneration by human dental pulp stem cells

通常，未分化的间充质细胞在人牙髓组织中供应丰富，并且已知含有大量干细胞。然而，牙髓干细胞（DPSC）的特性和分化信号的澄清仍然是未知的。因此，我们确定了CD44的表达，CD44被认为是人DPSC上的干细胞表面标志物。CD44表达约为60%。然后，作为CD44处理的配体的透明质酸诱导碱性磷酸酶的表达。这些数据表明，CD44信号传导可以诱导DPSC上的有效钙化。

In general, undifferentiated mesenchymal cells are in abundant supply in human dental pulp tissue, and it is known that containing a large number of stem cells. However, the clarification of characteristics of dental pulp stem cells (DPSCs) and differentiation signaling are still unknown. Therefore, We determined expression of CD44 that is known as a stem cell surface marker upon human DPSCs. The CD44 expression was approximately 60%. Then, hyaluronic acid that is a ligand of CD44 treatment induced expression of alkaline phosphatase. These data suggested that CD44 signaling may induce efficient calcification on DPSCs.