Plasma-Assisted Biological Macromolecular Crystallization

我们一直在研究等离子体辅助生物大分子结晶的新技术。用蛋清溶菌酶进行等离子体辐照可提高生物大分子的结晶率。为了了解脱色机理，我们研究了各种染料的脱色过程，通过染料的颜色变化来指示反应速度。反应速度在很大程度上取决于溶液的pH值，这种依赖关系并不是唯一的。结果表明，为了有效地应用新结晶技术，应适当考虑溶液条件。

We have been studying a new technique “plasma-assisted biological macromolecular crystallization”. Improvement in the crystallization probability of biological macromolecules by plasma irradiation had been reported with lysozymes from hen egg whites. For understanding the mechanisms, we studied decolorization process of various dyes indicating the reaction speed by their color change. The reaction speeds largely depend on pH of the solution and the dependency is not unique. The results suggest that, for useful application of the new crystallization technique, the conditions of solution should be considered properly.