Understanding of mechanisms underlying the expression changes of the therapeutic targets identified by quantitative proteomic analysis in renal cell carcinomas

在此之前，我们通过定量蛋白质组学分析检测了人肾细胞癌（RCC）癌组织和非癌组织蛋白质表达的差异，并鉴定了许多RCC相关蛋白。其中，磷酸果糖激酶同工酶和RNA结合蛋白的表达变化依赖于VHL状态，也发现在缺氧条件下，在人肾癌和结直肠癌细胞系。这些蛋白质在缺氧条件下在mRNA水平发生或不发生变化。同时，RCC患者和健康对照组之间的一些RCC相关蛋白的血浆浓度存在差异，这表明它们的使用可能是RCC的初步检测的有用工具。

Previously, we detected the differences in protein expression between cancerous and noncancerous tissues of human renal cell carcinomas (RCC) by quantitative proteomic analysis, and identified many RCC-related proteins. Among them, the expression changes of the isozymes of phosphofructokinase and one of RNA-binding proteins were dependent on VHL status and were also found under hypoxic conditions in human renal and colorectal cancer cell lines. Those of these proteins under hypoxia occurred with or without their changes at mRNA level. Meanwhile, there were differences in the plasma concentrations of some RCC-related proteins between RCC patients and healthy controls, suggesting their use might represent a useful tool for primary detection of RCC.