Multiple molecular imaging by EPR spectroscopy

电子顺磁共振(EPR)结合氮氧化物是显示体内氧化应激调节氧化还原状态的一种有效的、非侵入性的方法。然而，到目前为止，在使用氮氧化物的情况下，并不总是可以使用连续波(CW)-EPR成像仪在小型啮齿动物身上获得三维(3D)图像。由于成像仪的改进能够快速获取数据，用不同脂类的氮氧化物在小鼠身上测试了高质量3D EPR成像的可行性。利用改进的EPR成像仪，在体内进行了多分子成像研究。

Electron paramagnetic resonance (EPR) imagingcombined with nitroxides is a powerful, noninvasive method for visualizing the redox status modulated by oxidative stress in vivo. However, to date, it has not always been possible to obtain three-dimensional (3D) images with a continuous wave (CW)-EPR imager in small rodents while using nitroxides. Due to improvements in imagers that enable rapid data-acquisition, the feasibility of 3D EPR imaging with good quality in mice was tested with nitroxides of different lipophilicities. Using thisimproved EPR imager,multiple molecular imaging studies were carried out in vivo.