AtGenExpress: Ein multinationales Projekt zur Analyse des Transcriptomes der Modellpflanze Arabidopsis thaliana

使用ATH1全基因组芯片进行表达谱分析的Affyphin技术可以可靠地检测拟南芥中约24，000个蛋白质编码基因。它包括RNA处理和标记技术以及带有工作站和数据处理软件的基因阵列扫描仪。总而言之，这是一个独特的组合工具广泛使用的世界各地。基于这项技术，AFGN小组提出了一个国际合作项目（AtGenExpress），以生成一个基因表达谱数据库，该数据库涵盖来自500个RNA样本的约230个数据集，并得到DFG的财政支持。这部分包括拟南芥的发育、胁迫反应、光反应和病原菌互作的样本。将由NSF 2010年项目（美国）关于病原体反应的80组数据、RIKEN（日本）关于激素反应的170组数据以及图宾根和戈尔姆的多指标指数约50组数据作为补充。来自1300多个微阵列的整体数据库，即3000多万个数据点，将作为国际基因表达综合数据库（GEO）和ArraExpress数据库的一部分向公众发布。数据处理和出版将由NSF支持的拟南芥数据库（TAIR，USA）管理。

The Affymetrix technology for expression profiling with the ATH1 full genome chip allows the reliable detection of about 24,000 protein-coding genes of Arabidopsis. It includes the RNA processing and labelling technology and the Gene Array Scanner with workstation and data processing software. Alltogether this represents a unique combination of tools broadly used worldwide. Based on this technology, the AFGN groups propose an international cooperation project (AtGenExpress) to generate a gene expression profiling database covering about 230 data sets derived from 500 RNA samples with financial support by the DFG. This part includes samples for Arabidopsis development, stress responses, light response and pathogen interaction. It will be complemented by 80 data sets from the NSF 2010 project (USA) on pathogen responses, by 170 data sets from RIKEN (Japan) on hormone responses and by about 50 data sets from the MPIs in Tübingen and Golm. The overall data base derived from more than 1300 microarrays, i.e. more than 30 million data points, will be released to the public as part of the international Gene Expression Omnibus (GEO) and ArraExpress data bases. Data processing and publication will be managed by the NSF-supported Arabidopsis database (TAIR, USA).